Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPLHENLAGKTAVITGGSGVLCSAMARELARHGMKVAILNRTAEKGQAVVKEITAAGGTACAVAADVLDRMSLERAKEDILGQFGAVDLLINGAGGNHPDAITDVETYEEAGEGQSFFDMDERGFLTVFSTNFTGAFLASQVFGKELLKADSPAIINLSSMSAYSPMTKVPAYSAAKASINNFTMWMAVHFAETGLRVNAIAPGFFLTKQNHDLLINQDGTFTSRSHKIIAGTPMKRFGKPEDLLGTLLWLADESYSGFVTGITVPVDGGFMAYSGV 3UF0 Chain:A ((28-272)) ------SLAGRTAVVTGAGSGIGRAIAHGYARAGAHVLAWGRT-DGVKEVADEIADGGGSAEAVVADLADLEGAANVAEE-LAATRRVDVLVNNAG------IIARAPAEEVSLGR---------WREVLTVNLDAAWVLSRSFGTAMLAHGSGRIVTIASMLSFQGGRNVAAYAASKHAVVGLTRALASEWAGRGVGVNALAPGYVVTANTAALRADDE-----RAAEITARIPAGRWATPEDMVGPAVFLASDAAS-YVHGQVLAVDGGWLA----

General information: TITO was launched using: RESULT: Template: 3UF0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1377 -151835 -110.27 -619.73 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -110.27 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_3UF0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UF0-query.scw PDB file : Tito_Scwrl_3UF0.pdb: