Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSELLATYLLT-EPGADTEKKAEQIATGLTVGSWTDLPLVKQEQMQKHKGRVIKVEEREGTAASEKQAVITIAYP-----EINFSQDIPALLTTVFGKLSLDGKIKLIDLHFSEAFKRALPGPKFGVYGIRKLLGEFERPLLMSIFKGVIGRDLSDIKEQLRQQALGGVDLIKDDEIFFETGLAPFETRIAEGKQILKETYEQTGHKTLYAVNLT-GRTADLKDKARRAAELGADALLFNVFAYGLDVMQGLAE---DPEIPVPIMAHPAVSGAFTSSPFYGFSHALLLGKLNRYCGADFSLFPSPYGSVALPRADALAIHEECVREDA----------FNQTFA-------VPSAGIHPGMVPLLMRDFGIDHIINAGGGVHGHPNGAQGGGRAFRAIIDAVLEAQPIDEKAEQCKDLKLALDKWGKAEAV 1AUS Chain:L ((34-431)) -TDILAAFRVSPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTNLDRYKGRCYHIEP---VAGEENQYICYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRA-LRLEDLRIPVAYVKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCAEALYKAQAETGEIKGHYLNATAGTCEDMMKRAVFARELGVPIVMHDYLTGGFTANTTLSHYCRDNGLLLHI--HRAMHAVIDRQKNHGM-HFRVLAKALRLSGGDHIHSGTVV-----ERDITLGF-VDLLRDDYTEKDRSRGIYFTQSWVSTPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVQAR-------------------------

General information: TITO was launched using: RESULT: Template: 1AUS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 1983 -203181 -102.46 -555.14 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain L : 0.72 3D Compatibility (PKB) : -102.46 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_1AUS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AUS-query.scw PDB file : Tito_Scwrl_1AUS.pdb: