Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHLLNPKAREIEISGIRKFSNLVAQHEDVISLTIGQPDFFTPHHVKAAAKKAIDENVTSYTPNAGYLELRQAVQLYMKKKADFNYDAESEIIITTGASQAIDAAFRTILSPGDEVIMPGPIYPGYEPIINLCGAKPVIV--DTTSHGFKLTARLIEDALTPNTKCVVLPYPSNPTGVTLSEEELKSIAALLKGRNVFVLSDEIYSELTYDRPHYSIATYLRDQTIVINGLSKSHSMTGWRIGFLFAPKDIAKHILKVHQYNVSCASSISQKAALEAVT----NGFDDALIMREQYKKRLDYVYDRLVSMGLDVVKPSGAFYIFPSIKSFGMTSFDFSMALLEDAGVALVPGSSF--STYGEGYVRLSFACSMDTLREGLDRLELFVLKKREAMQTINNGV 1V2F Chain:A ((3-368)) ----LHPRTEAAKESIFPRMSGL-AQRLGAVNLGQGFPSNPPPPFLLEAVRRALGRQ-DQYAPPAGLPALREAL------AEEFAVEPES-VVVTSGATEALYVLLQSLVGPGDEVVVLEPFFDVYLPDAFLAGAKARLVRLDLTPEGFRLDLSALEKALTPRTRALLLNTPMNPTGLVFGERELEAIARLARAHDLFLISDEVYDELYYGERPRRLREFAPERTFTVGSAGKRLEATGYRVGWIVGPKEFMPRLAGMRQWTSFSAPTPLQAGVAEALKLARREGFYEAL--REGYRRRRDLLAGGLRAMGLRVYVPEGTYFLMAELP--GWDAFR----LVEEARVALIPASAFYLEDPPKDLFRFAFCKTEEELHLALERLGRVV--------------

General information: TITO was launched using: RESULT: Template: 1V2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1986 -254372 -128.08 -710.53 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -128.08 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_1V2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V2F-query.scw PDB file : Tito_Scwrl_1V2F.pdb: