Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERFEITKTPINTENIIKKVEKREAGAITTFIGTVREWTNGKRTVRLEYEAYEPMAVQMLAQIGAEIEEKWEGASAAITHRIGVLDIGEAAVVIAVSSPHRKAAYEANEYAIERIKQIVPIWKKEIWEDGEQWIGDQLETTAYPNGKPDLSEGEQHD 2QIE Chain:H ((1-136)) MKQFEIVIEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKTEYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRKDAYRANEYAIERIKEIVPIWKKEI-----KWQGH---------------------

General information: TITO was launched using: RESULT: Template: 2QIE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 605 -72211 -119.36 -551.23 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain H : 0.82 3D Compatibility (PKB) : -119.36 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_2QIE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QIE-query.scw PDB file : Tito_Scwrl_2QIE.pdb: