Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTFVKKAM-LTTAAMTSAALLTFGPDAASAKTPVDNTAVQLQHQASTNEDLNTFIDILNQCIYEQDGVYYFDSEKAVELGMTKEEAQVIATLWESTSEFFSIVSQCVYLEDGN---------YKFDTEKAVELGFT--EKEALALEQFFSAVSLKIHIL----QAAIVLQDDVYSYDKDAALQAGATPLQADVYEK-LFSALSQEQLAAIYDMIHPQA 2W69 Chain:A ((7-203)) AMEDFVRQCFNPMIVELAEKAMKEYGED----------LKIETNKFAAICTHLEVCFMYSDFHFINEQG-----ESIVVELDDPNALKHRFEIIEGRDRTMAWTVVNSICNTTGAEKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQEMANRGLWDSFRQSER-----------

General information: TITO was launched using: RESULT: Template: 2W69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 669 -56886 -85.03 -321.39 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -85.03 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_2W69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W69-query.scw PDB file : Tito_Scwrl_2W69.pdb: