Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYLDVIWLLNFCFDALLLLLTAFILKRHVKKRRLVGGAFIGSSIVLLMFTPFSPIVEHPAGKLAFSVVIVVVTFGFKRFRFFFQNLFSFYFATFLMGGGIIGAHSLLQSNSIVQNGVMITNQTGFGDPISWLFIVGGFPALWFFSKRRIEDIETKNIQYEERVSVQADLGSQTLHVRGLIDSGNQLYDPLTKTPVMIIYIDKLEPIFGTAETMIIRNTDPLEAIEQLDDSFRFLDKMRLIPYRGVGQQNQFLLCVKPDHVTIMTKEEMISADKCLIGISTTKLSADGEFDAIIHPKMLSGKAVKHVS 5C9F Chain:A ((20-53)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------HFYINAFVNNV--KIKFMVDTGASDI---------ALTKEDAQKL-----------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5C9F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 66 -8721 -132.14 -256.50 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.45 3D Compatibility (PKB) : -132.14 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.45 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_5C9F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C9F-query.scw PDB file : Tito_Scwrl_5C9F.pdb: