Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRNKRERQELLQQTIQATPFITDEELAGKFGVSIQTIRLDRLELSIPELRERIKNVAEKTLEDEVKSLSLDEVIGEIIDLELDDQAISILEIKQEHVFSRNQIARGHHLFAQANSLAVAVIDDELALTASADIRFTRQVKQGERVVAKAKVTAVEKEKGRTVVEVNSYVGEEIVFSGRFDMYRSKHS 2F3X Chain:B ((13-155)) -------------------------------------------ELSIPELRERIKNVAEKTLEDEVKSLSLDEVIGEIIDLELDDQAISILEIKQEHVFSRNQIARGHHLFAQANSLAVAVIDDELALTASADIRFTRQVKQGERVVAKAKVTAVEKEKGRTVVEVNSYVGEEIVFSGRFDMYRSK--

General information: TITO was launched using: RESULT: Template: 2F3X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 682 -56276 -82.52 -393.54 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain B : 0.90 3D Compatibility (PKB) : -82.52 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_2F3X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F3X-query.scw PDB file : Tito_Scwrl_2F3X.pdb: