Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGDSILFTVKNEHMALITLNRPQAANALSAEMLRNLQMIIQEIEFNSNIRCVILTGTGEKAFCAGADLKERIKLKEDQVLESVSLIQRTAALLDALPQPVIAAINGSALGGGLELALACDLRIATEAAVLGLPETGLAIIPGAGGTQRLPRLIGRGKAKEFIYTGRRVTAHEAKEIGLVEHVTAPCDLMPKAEELAAAISANGPIAVRQAKFAINKGLETDLATGLAIEQKAYEQTIPTKDRREGLQAFQEKRRAVYKGI 4FJW Chain:D ((10-250)) ---------RDQRVGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSA-KAFAAGADIKEMADLTFADAFTADFFA--TWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADTAKFGQPQIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADDLLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAFATEDQSEGMAAFIEKR-------

General information: TITO was launched using: RESULT: Template: 4FJW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1153 -120169 -104.22 -498.63 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -104.22 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_4FJW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FJW-query.scw PDB file : Tito_Scwrl_4FJW.pdb: