Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVIHNGKAGLLGLSVQDVPSTKPGYGEVKVKLKSAGLNHRDL------------FLMKNKSEQD-------PHMILGSDGAGIIEEIGEGVKNVTVQTEVVIFPTLNWDLTEN-------VPPVPEILGGPSD-GTLAEYVIIPSQNAIKKPAYLSWEEAGVLPLSALTAYRALFTK--GQLKKGEHLLIPGIGSGVATYALFMAKAIGATVSVTSRSEEKRKKALKLGADYAFDS----YSNW-DEQLQ-----------------GKKIDVVLDSIGPALFSEYFRHVKPNGRIVSFGASSGDNLSFPVRSLFFPQVNVLGTSMGSGEEFQAMLAFIDKHKLRPVIDRIYPLEKACEAYKRMQEGRQFGNIGIVME 3KRT Chain:A ((58-421)) --------------IHLDDVPVPELGPGEALVAVMASSVNYNSVHTSIFEPLSTFGFLERYGRVSDLAKRHDLPYHVIGSDLAGVVLRTGPGVNAWQAGDEVVAH-CLSVELESSDGHNDTMLDPEQRIWGFETNFGGLAEIALVKSNQLMPKPDHLSWEEAAAPGLVNSTAYRQLVSRNGAGMKQGDNVLIWGASGGLGSYATQFALAGGANPICVVSSPQKAEICRAMGAEAIIDRNAEGYRFWKDENTQDPKEWKRFGKRIRELTGGEDIDIVFEHPGRETFGASVFVTRKGGTITTCASTSGYMHEYDNRYLWMSLKRIIGSHFANYREAWEANRLIAKGRIHPTLSKVYSLEDTGQAAYDVHRNLHQGKVGVLC-

General information: TITO was launched using: RESULT: Template: 3KRT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1725 21160 12.27 67.60 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 12.27 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_3KRT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KRT-query.scw PDB file : Tito_Scwrl_3KRT.pdb: