Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MNFLLDLFTNWTFDKVLDYTLAAVI-------WSVFKSRSKQ---NKYPGYFEKIRRYRN---LHPLLRSL---SMRVLLSITIHPYMFS---------------------------------------------------------------------------- 4EI7 Chain:A ((3-384)) GNFSEIESQGNISLKFGFLGLGMGGCAIAAECANKETQIKNNKYPYRAILVNTNSQDFNKIEIKNTGNVRKIQLEGYEQGAARNPQVGEEAFVKHETKIFEAVKQEFEDRDFIWITCGLGGGTGTGALLKAIEMLYEHDYNFGLLLTLPRDAEALKVLENATSRIRSIAMNQEAFGSIVLIDNAKLYRKFEEENPSALANEYTSYSNKYIADALHEINLVTSSFTPFSDTHFDASEFAQVINTPGVLSLAKLELKSNQLDTENPLGYLTQLGNALEKGVLYDTEREELESAKKSALSIVTSPLRAGRLYNFSFLNQMENFLKERTPYVDERPIAPYVNKHTTKKEEDIVKFYSVVAGLPLPKRVSDIIDEITRIKEEREQAN

General information: TITO was launched using: RESULT: Template: 4EI7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 285 11898 41.75 160.78 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 41.75 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.049

(partial model without unconserved sides chains): PDB file : Tito_4EI7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EI7-query.scw PDB file : Tito_Scwrl_4EI7.pdb: