Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMLEQLIQLKQDLIDGSKVEKPSLDDKQIDEMDILVSEALEFNKELKFKLFNKGFVENVTGRVHYINFEQQKLHVKDQNDNTVYINMNNIIRVIYND 3SB2 Chain:A ((11-49)) ---------------------------------PFLNALRKEHVPVSIYLVNG---IKLQGHVESFD--QYVVLLRN--------------------

General information: TITO was launched using: RESULT: Template: 3SB2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 90 -14065 -156.27 -360.63 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -156.27 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.686

(partial model without unconserved sides chains): PDB file : Tito_3SB2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SB2-query.scw PDB file : Tito_Scwrl_3SB2.pdb: