Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLIKLEQPNCNPCKMVSNYLEQVNIQFET------VDVTQEPEVAARFGVMGVPVTILLSDQGEEVNRSVGFKPNELDELLKELR 2L4Q Chain:A ((29-108)) --LVDFWATWCGPCKMVAPVLEEIATERATDLTVAKLDVDTNPETARNFQVVSIPTLILFKD-GQPVKRIVGAKGKA--ALLREL-

General information: TITO was launched using: RESULT: Template: 2L4Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 258 -2511 -9.73 -33.93 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -9.73 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_2L4Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L4Q-query.scw PDB file : Tito_Scwrl_2L4Q.pdb: