Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNKIYDWVDERLDITPMWRDIADHEVPEHVNPAHHFSAFVYCFGGLTFFVTVIQVLSGMFLTMYYVPDIKNAWESVYYLQNEVAFGQIVRGMHHWGASLVIVMMFLHTLRVFFQGAYKKPRELNWIVGVLIFFVMLGLGFTGYLLPWDMKALFATKVGLQIAEATPLIGTQVKTLLAGHPDIVGAQTLTRFFAIHVFFLPAALFGLMAAHFIMIRKQGISGPL 2QJK Chain:A ((13-222)) ----IEKWLHSRLPIVALAYDTIMIPTPRNLN-------WMWIWGVVLAFCLVLQIVTGIVLAMHYTPHVDLAFASVEHIMRNVNGGFMLRYLHANGASLFFIAVYLHIFRGLYYGSYKAPREVTWIVGMLIYLAMMATAFMGYVLPWGQMSFWGATVITGLFGAIPGIGHSIQTWLLGGPAVDNA-TLNRFFSLH-YLLPFVIAALVAIHIWAFHSTGNNNP-

General information: TITO was launched using: RESULT: Template: 2QJK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 676 -124406 -184.03 -592.41 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -184.03 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_2QJK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QJK-query.scw PDB file : Tito_Scwrl_2QJK.pdb: