Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMDFNLFMNDIVRQARQEITAAGYTELKTAEEVDEALT--KKGTTLV-MVNSVC-GCAGGIARPAAYHSVHYDKRPDQLVTVFAGQDKEA--TARARDYFEGYPPSSPSFAILKDGKIMKMVERHEIEGHEPMAVVAKLQEAFEEYCEEV 2PU9 Chain:C ((7-109)) ------------------------VTEV-NKDTFWPIVKAAGDKPVVLDMFTQWCGPSKAM--APKYEKLAE--EYLD---VIFLKLDCNQENKTLAKEL--G-IRVVPTFKILKENSVVG-----EVTGAKYDKLLEAIQAA--------

General information: TITO was launched using: RESULT: Template: 2PU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 403 -567 -1.41 -5.85 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : -1.41 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_2PU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PU9-query.scw PDB file : Tito_Scwrl_2PU9.pdb: