Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDPSTLMILAIVAVAIIVLAVFFTFVPVMLWISALAAGVKISIFTLVGMRLRRVIPNRVVNPLIKAHKAGLNVGTNQLESHYLAGGNVDRVVNALIAAQRANIELTFERCAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMDGIEVKAKARITVRANIERLVGGAGEETIVARVGEGIVSTIGSSDNHKKVLENPDMISQTVLGKGLDSGTAFEILSIDIADVDIGKNIGAILQTDQAEADKNIAQAKAEERRAMAVAQEQEMRARVEEMRAKVVEAEAEVPLAMAEALREGNIGVMDYMNIKNIDADTEMRDSFGKLTKDPSDEDRKS 5A5B Chain:W ((50-167)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GLISGAGANPRVLSTFTAEFGK-ILAGLHDTQIEGKLHMATALQIAQLTLKHRQNKVQHQRIVAFVCSPISDSRDELIRLAKTLKKNNVAV-DIINFGEIEQNTELLDEFIAAVNNPQEE----

General information: TITO was launched using: RESULT: Template: 5A5B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain W - contact count / total energy / energy per contact / energy per residue : 402 -17376 -43.22 -147.25 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain W : 0.55 3D Compatibility (PKB) : -43.22 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.285

(partial model without unconserved sides chains): PDB file : Tito_5A5B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5A5B-query.scw PDB file : Tito_Scwrl_5A5B.pdb: