Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKTDKLLESLNVFIQKAEENQYKQLGEMVPDFPGKSNIPKYVEEYEKGIARLLRRQHKKFLDGLKGFVSKDSEETLKALLVFFTQNLFAEDDFEEEFQELTEGFLQQTIEEMAEVIMDSIDPEVPFKVLSNRTINWIKDWSKKLAEIMKLNTHEVVEDVLTNAIENGSSIQDIELTLKDMPQFDRERARTTAITEVLAASSAAQHESYAQSPAVKKKKWRHSGGKKNNPRENHIDLDGTVIGVDEEFQIPGSSETCMFPRDPKLSTRERVNCHCVLSPVVDSKILGLSPEEKEEIQREVLANME 2HL7 Chain:A ((42-73)) ------------------------------------------------------------------------------------------------------------------------------------------------------APIAADLRKQIYGQLQQGKSDGEIVDYMVARY---------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2HL7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 43 -6321 -147.00 -197.53 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -147.00 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.876

(partial model without unconserved sides chains): PDB file : Tito_2HL7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HL7-query.scw PDB file : Tito_Scwrl_2HL7.pdb: