Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKIIIDGRDFENIEVLHDDLKDKLDFPDYYGRNLDALWDCLTGWVDLPLTLVLKNFEFSNTFLGSYADDVLEVIQEAQEELKDEFKIIIE 1X1X Chain:D ((1-83)) -KKAVINGEQIRSISDLHQTLKKELALPEYYGENLDALWDALTGWVEYPLVLEWRQFEQSKQLTENGAESVLQVFRAAKAEGAD-------

General information: TITO was launched using: RESULT: Template: 1X1X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 322 -44181 -137.21 -532.30 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -137.21 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_1X1X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1X1X-query.scw PDB file : Tito_Scwrl_1X1X.pdb: