Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRILGLDLGTKTLGVALSDEMGWTAQGIETIKINEAEGDYGLSRLSELIKDYTIDKIVLGFPKNMNGTVGPRGEASQTFAKVLETTYNVPVVLWDERLTTMAAEKMLIAADVSRQKRKKVIDKMAAVMILQGYLDSLN 1NU0 Chain:A ((3-137)) GTLMAFDFGTKSIGVAVGQRITGTARPLPAIKAQD--GTPDWNIIERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVEA-------GGYRALNKGKVDSASAVIILESYMEQG-

General information: TITO was launched using: RESULT: Template: 1NU0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 554 -66039 -119.20 -515.93 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -119.20 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_1NU0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NU0-query.scw PDB file : Tito_Scwrl_1NU0.pdb: