Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRFSTAYLLLGILCSAAVFLIGAPSRALGAEVEHYEPLQVHVQLEKVYLDGDVSIEHKHEKVFSMDDFWAAYAGWTLVEQKKGYVLFRKQMDDISPLSKVNGYIGVSDNGVISTFHGRPEPASEPIQSFFQIDLERLESHMQKNLLKGIPFRTKAEFEDVIEHMKTYSG 2HBP Chain:A ((21-57)) --------------------------------------------------------------------------------------------------------FIGCAKGKIHLHKAN--G--V----KIAVAADKLSNEDLAYVEKI---------------------

General information: TITO was launched using: RESULT: Template: 2HBP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 89 -16536 -185.80 -446.92 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : -185.80 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.840

(partial model without unconserved sides chains): PDB file : Tito_2HBP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HBP-query.scw PDB file : Tito_Scwrl_2HBP.pdb: