Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGRTLFFRRLFDYYKYQFKVLHAVIDWTVALYIVLPAIAFVIYQYIDLMNGRGLLYEWSE-VAEWRWLYAVCVLIMFTGSIRTFLMEADK-----VFLLQKKEI--IYQLKRYALLYSFLATLAKW--LLLFFI------VLPLISHSV---------------------LITFAESTALLCYLFGLHIFFLSLKQDRIRKPRSISRWIGDTLVRAILFAG-SAILIVFTERHLLALF-----GILFLFFSVIRSLKKTASFTA-FEAEVTEEKKSRLALA---GLVMMMSQEAGMPKVKDRMRRKPLLYRNSKRIFKRRTICTGYKELFFKVMLRNGEYARQMYMLLSAFTVLIFVSPIWLKVIALLVYTGVCRYILTLIFDKVMDAPFLIGTDKESDEYYRARKSCINILHYAFAACCFLAAAVSLLFT--- 4ZOX Chain:A ((53-431)) MSNNSL---TYFDKHTDSVFAIGHHPNLPLVCTGGGDNLAHLWTSHSQPPKFAGTLTGYGESVISCSFTSEGGFLVTADMSGKVLVHMGQKGGAQWKLASQMQEVEEIVWLKTHPTIARTFAFGATDGSVWCYQINEQDGSLEQLMSGFVHQQDCSMGEFINTDKGENTLELVTCSLDSTIVAWNCFTGQQLFKITQAEIKGLEA--PWISLSLAPETLTKGNSGVVACGSNNGLLAVINCNNGGAILHLSTVIELKPEQDELDASIESISWSSKFSLMAIGLVCGEILLYDTSAW------RVRHKFVLEDS-----------------VTKLMFDND---------------DLFASCINGKVYQFNARTGQEKFVCVGHNMGVLDFILLHPVANTGTEQKRKVITAGD-------------EGVSLVFEVPN

General information: TITO was launched using: RESULT: Template: 4ZOX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1976 -173481 -87.79 -527.30 target 2D structure prediction score : 0.19 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -87.79 2D Compatibility (Sec. Struct. Predict.) : 0.19 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.111

(partial model without unconserved sides chains): PDB file : Tito_4ZOX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZOX-query.scw PDB file : Tito_Scwrl_4ZOX.pdb: