Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIDSWLNDRLDIAKEAGVHRNLRSMNGAPVPERNIDGENQTVWSSNNYLGLASDRRLIDAAQTALQQFGTGSSGSRLTTGNSVWHEKLEKKIASFKRTEAALLFSSGYLANVGVLSSLPEKEDVILSDQLNHASIIDGCRLSKADTVVYRHIDMNDLENKLNETQRYQRRFIVTDGVFSMDGTIVPLDQIISLAKRYHAFVVVDDAHATGVLGDSGRGTSEYFGVYP--DIVIGTLSKAVGTEGGFAAGSAVFIDFLLNHARTFIFQTAIPPASCA----------AAHE--------AFNIRTSLKNMGYVVKGD--HTPIIPVVIGDAHKTVIFAEKLQGKGIYAPAIRPPTVAPGESRIRITITSDHSMGDIDDLLKTFHSIGKELHII 2W8V Chain:A ((59-419)) --------------------------------EAVIRGKDTILLGTYNYMGMTFDPDVIAAGKEALEKFGSGTWGSRMLNGTFHDHMEVEQALRDFYGTTGAIVFSTGYMANLGIISTLAGKGEYVILDADSHASIYDGCQQGNAEIVRFRHNSVEDLDKRLGRLPKEPAKLVVLEGVYSMLGDIAPLKEMVAVAKKHGAMVLVDEAHSMGFFGPNGRGVYEAQGLEGQIDFVVGTFSKSVGTVGGFVVSNHPKFEAVRLACRPYIFTASLPPSVVATATTSIRKLMTAHEKRERLWSNARALHGGLKAMGFRLGTETCDSAIVAVMLEDQEQAAMMWQALLDGGLYVNMARPPATPAGTFLLRCSICAEHTPAQIQTVLGMFQAAGRAVGVI

General information: TITO was launched using: RESULT: Template: 2W8V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1953 -126470 -64.76 -373.07 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -64.76 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_2W8V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W8V-query.scw PDB file : Tito_Scwrl_2W8V.pdb: