Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTEQPNWLMQRAQLTPERIALIYEDQ--TVTFAELFAASKRMAEQLAAHSVRKGDTAAILLQNRAEMVYAVHACFLLGVKAVLLNTKLSTHERLFQLEDSGS--------GFLLTDSSFEKKEYEHIVQTIDVDELMKEAAEEIEIEAYMQMDA-TATLMYTSGTTGKPKG--VQQTFGNHYFSAVSSALNLGITEQDRWLIALPLFHISGLSA-LFKSVIYGMTVVLHQRFSVSDVLHSINRHEVTMISAVQTMLASLLEETNRCP-----ESIRCILLGGGPAPLPLLEECREKGFP--VFQSYGMTETCSQIVTLSPEFSMEKLGSAGKPLFSCEIKIERDG----QVCEPYEHGEIMVKGPN-VMKSYFNRESANEASFQNGWLKTGDLGYLDNEGFLYVLDRRSDLIISGGENIYPAEVESVLLSHPAVAEAGVSGAEDKKWGKVPHAYLV--LHKPVSAGELTDYCK-ERLAKYKIPAKFFVLDRLPRNASNKLLRNQLKDARKGELL 1T5H Chain:X ((8-498)) --------LRRAATRAPDHCALAVPARGLRLTHAELRARVEAVAARLHADGLRPQQRVAVVAPNSADVVIAILALHRLGAVPALLNPRLKSAE-LAELIKRGEMTAAVIAV--QVADAIFQSGSGARIIFLGDLVRDGEPYSYGPPIEDPQREPAQPAFIFYTS---GLPKAAIIPQRAAESRVLFMSTQVGLRHGRHNVVLGLMPLYHVVGFFAVLVAALALDGTYVVVEEFRPVDALQLVQQEQVTSLFATPTHLDALAAAAAHAGSSLKLDSLRHVTFAGATMPDAVLETVHQH-LPGEKVNIYGTTEAMNSLYMRQP-----KTGTEMAPGFFSEVRIVRIGGGVDEIVANGEEGELIVAASDSAFVGYLNQPQATAEKLQDGWYRTSDVAVWTPEGTVRILGRVDDMIISGGENIHPSEIERVLGTAPGVTEVVVIGLADQRWGQSVTACVVPRLGETLSADALDTFCRSSELADFKRPKRYFILDQLPKNALNKVLRRQL---------

General information: TITO was launched using: RESULT: Template: 1T5H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 2544 -32893 -12.93 -71.97 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain X : 0.75 3D Compatibility (PKB) : -12.93 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_1T5H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T5H-query.scw PDB file : Tito_Scwrl_1T5H.pdb: