Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSKLSILMIGFALSVLLAACGSNDAKEEKTDTGSKTEATASEGEELYQ-QSCVGCHGKDLEGVSGPNLQEVGGKYD-----EHKIESIIKNGRG------NMPKGLVDDNEAAVIAKWLSEKK 2I8F Chain:A ((2-78)) ------------------------------------------DGEALFKSKPCAACHSVDTKMV-GPALKEVAAKNAGVEGAADTLALAIKNGSQGVWGPIPMPPNPVTEEEAKILAEWV----

General information: TITO was launched using: RESULT: Template: 2I8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 163 13322 81.73 204.95 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 81.73 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_2I8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I8F-query.scw PDB file : Tito_Scwrl_2I8F.pdb: