Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MYKADYKQIAATPSFQAFLKQKRAFIVPSAIFFFVFYFSL--PVLTSYFTFLNAPAIGAVSW-AWLFAI-----------AQFAMTWILSTV-----YSRRAAHFDKYVSALKEDLKGEQT 2LGE Chain:A ((1-129)) GDDDEPGGKGAMYEVTIEQS---GDFRSFIKSVVVVANGTQLKDGATGESLASPVILSDEELAVEKVTLSTTGKAIEFAVSGGVVDGEDGVVNEPMQWVVTVYKNGKEIEKKSLVFRDGKEISTDDLNLYYN

General information: TITO was launched using: RESULT: Template: 2LGE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 379 -18526 -48.88 -187.13 target 2D structure prediction score : 0.08 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -48.88 2D Compatibility (Sec. Struct. Predict.) : 0.08 1D Compatibility (Hydrophobicity) : 0.67 QMean score : -0.027

(partial model without unconserved sides chains): PDB file : Tito_2LGE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LGE-query.scw PDB file : Tito_Scwrl_2LGE.pdb: