Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHKEIAKELLLLAGGKNNIISISHCTTRLRFDVKDETKIDIHAIENLQGVQGTFFRYGLFQIIFGAGVVNKIYKEVVHVWETAPSEEPVHQKKASRKLNPAAAFAKTLSDIFVPIIPAITASGLLMGLIGMIKVF--HWFA--AGSPWIKMLDLVSSTAFILLPIL----VGFSAARQFGSNPYLGAVIAGL---LTHPDLLDPSMLGSKTPSSLDIWGLHIPMMGYQGSMIPILLSVFVMSKIEKLLKS-----IVPKSLDV-------VIIPFITVM-VTGCLALIVMNPAASIIGQIMTQSI-VYIYD-HAGIAAGALFGGIYSTIVLSGLHHSFY--AIE----ATLLANPH----VGVN---FLV-PIWS-MANVAQGGAGL----AVFLKTKQSSLKKIALPASLTAFLGIVEPIVFGVNLKLI----RPFIGAAIGGAIGGAYVVAVQVVANSYG-LTGIPMIS-IVLP----FGAANFVHYMIGFLIAAVSAFIATL-FLGFKEETE 3QNQ Chain:A ((12-423)) ----------------------------------------------------------------------------------------------VAGKVAEQRHLLAIRDGLVLTMPFLIIGSIFLIISTLPIPGYSEFMASLFGKNWNVALGYPVSATFNIMALIAVFGIAYRLGEYYKVDALASGALSLVTFLLATPFQVAY-IMPGTKESILVDGVIPAA-LMGSQGLFVAMIIAIISTEIYRFLVQKKMIIKMPETVPPAVTRSFAALIPGFIVVTVVWIIRLIFEHTTFGSIHNVVGKLLQEPLSILGASLWGAVIAVILVHVLWACGIHGATIVGGVMSPIWLSLMDQNRIAFQAGQDVPNTITAQFFDLWIYMGGSGATLALVVGMLLFARSQQLKSLGRLSIAPGIFNINEMVTFGMPIVMNPLLLIPFIVVPVVLTIVSYFAMEWGL----VARPSGAAVTWTTPILFSGYLGSGGKISGVILQLVNFALAFVIYLPFLKIWD---

General information: TITO was launched using: RESULT: Template: 3QNQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1566 -188598 -120.43 -529.77 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -120.43 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.193

(partial model without unconserved sides chains): PDB file : Tito_3QNQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QNQ-query.scw PDB file : Tito_Scwrl_3QNQ.pdb: