Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKIKDDCIELELTPRRYQELDDDPFILSVFELLENKKAVVRDFSAVLLESEYKVLISGIETMIKGNQDSISLETIEPFLFLSIDQENGNYRIKIKIIFDDYKESKSNSNLFEINCNEEKLESFVTA-LKLNLENTKNPSKLP 2ZFD Chain:B ((34-120)) ----------------------------------------ERFVS----AWTAERVVERLEEIVSAENLTVAK--KETW-GMKIEGQKGNFAMVVEINQL--TDELVMIEVRKRQRAA---RDLWTDTLRPFFVEL-------

General information: TITO was launched using: RESULT: Template: 2ZFD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 333 -32936 -98.91 -396.82 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : -98.91 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_2ZFD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZFD-query.scw PDB file : Tito_Scwrl_2ZFD.pdb: