Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKVHQSACPLNCWDSCGFLVTVDDGKVTKVDGDPNHPITEGKICGRGRMLETKTNSPDRLRYPMKK-------------QNGEF--VRISWEQALDEIADKLREIKETSETTAVLHSHD--YANNGLLKALD---QRFFNGYGGVTEIVGSICWGSGIEAQSWDFGRS--YGHG---PLDIYNSKHVVVWGRNVSRTNMHL---------YHHLQQVKKK----GATITVIDPIFNPT-AKLADRYISVKPGMDGWLAAAVLKVLIEMGRTDETFISEHSVGFDDVKELL--------KTVSLEEFIVKTETSMEELEYLAGLYADGPVSTFMGLGMQRYKNGGGTIRWIDALVAAS-GNVGIKGGGANFGN----VQIGESFAKTKLTLP--------------ELKTTSRSFSMMTQAEEVLTAAD-----------PAIEMIIVTCGNPLTQVPNTNKVRQAFEKVPMTVAIDSIMTDTAELCDYVLPTATVFEEEDIYYSSMYHHYVQYG----KKLVEPQGEAKSDSWIWSELAKRLGFGELFEYSTQ----EFLEMGLSSLEAEDV------TLERLKEKGHLPLPVKQVPWDDYQFL----------TPSGKFEFTSSLAEQKGFSGS----LQLNVPEESVFHNEELAGKYPYTLLSIHPQRSNHSQHVPFIEKLQHVQVD------ISPDIAAGQDLQDGDEVVIFNDRGSMKGKVKVMKQAHAKTINIDEGMWAAFGGSVNALTNDTNSDNGMGSTLFDCLVGLKKA 1TMO Chain:A ((84-758)) --------------------------------------------------------NPSRVRYPMVRLDFLLKGHKSNTHQRGDFRFVRVTWDKALTLFKHSLDEV-QTQYGPSGLHAGQTGWRATGQLHSSTSHMQRAVGMHGNYVKKIGDYSTGAGQTILPYVLGSTEVYAQGTSWPLILEHSDTIVLWS-NDPYKNLQVGWNAETHESFAYLAQLKEKVKQGKIRVISIDPVVTKTQAYLGCEQLYVNPQTDVTLMLAIAHEMISKKLYDDKFIQGYSLGFEEFVPYVMGTKDGVAKTPEWAAPICGVEAHV--IRDLAKTLVKGRTQFMMGWCIQRQQHGEQPY-WMAAVLATMIGQIGLPGGGISYGHHYSSIGVPSSGAAAPGAFPRNLDENQKPLFDSSDFKGASSTIPVARWIDAILEPGKTIDANGSKVVYPDIKMMIFSGNNPWNHHQDRNRMKQAFHKLECVVTVDVNWTATCRFSDIVLPACTTYERNDI---DVYGAYANRGILAMQKMVEPLFDSLSDFEIFTRFAAVLGKEK--EYTRNMGEMEWLETLYNECKAANAGKFEMPDFATFWKQGYVHFGDGEV-WTRHADFRNDPEINPLGTPSGLIEIFSRKIDQFGYDDCKGHPTWMEKTERS--HGGPGSDKHPIWLQSCHPDKRLHSQMCESREYRETYAVNGREPVYISPVDAKARGIKDGDIVRVFNDRGQLLAGAVVSDNFPKGIVRIHEGAW----------------------------------

General information: TITO was launched using: RESULT: Template: 1TMO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2864 39930 13.94 70.80 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 13.94 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_1TMO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TMO-query.scw PDB file : Tito_Scwrl_1TMO.pdb: