Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQPQAFYRVVAEDFTAVDGIIRRQLTSRVPLVEKIGDYIISAGGKRLRPLLVLLAGKTLGYKGDDLCLLA--ATIEFLHTSTLLHDDVVDASGLRRGRSTANALWGNAPSVLVGDFLYARSFEMMVELGS-MPVMRIISQATRVIAEGEVLQLSKVRDASTTEETYMEVIRGKTAMLFEASTHSAAALCQAGEEQSEALRRFGDYLGIAFQLVDDLLDYRGDAATLGKNVGDDLAEGKPTLPLIVTMRDGTEEQAALVRKAIQQGGSQDLESVCAAVEAAGALDYTANLARDYAARAIACLDTLPDNEYRSALVELSEFAVARTH 3PKO Chain:A ((30-333)) -----------------DYLLRTVQLDNQPIHHKILA-LLKSGGKLLRPGYFYLFS-TFGNAATPAQLQAGAAAIEILHVGTLIHDDVIDDSPTRRGVRTIQMTYGQRNAIYAGDFMFTVYFDQVLKSTTDRSLIQNHIDAMHRILQGELHQMDLNYREDITLDAYLNEIAGKTAELFALSCYQGAQLAGAPQSVIDRTRDIGIAIGCAYQMLDDILDYAGDPKRTQKPVLEDLRSGVYSLPLLLSLSHAPRDFHKLLKKK-QAMTLEDIKHVQALVAQYDGVGAAKQLAQDYTDRALTLIQQLPVGSAQQSLEQLTRLLLRRD-

General information: TITO was launched using: RESULT: Template: 3PKO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -136394 for 2444 contacts (-55.8/contact) + 2D Compatibility (PS) -32963 + (NN) -13293 + (LL) 2256 1D Compatibility (HY) -17200 + (ID) 4700 Total energy: -202294.0 ( -82.77 by residue) QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_3PKO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PKO-query.scw PDB file : Tito_Scwrl_3PKO.pdb: