Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METIIIALIAFIIGIIAIPIVLFAWIYIKDEKQQEHSILRNYPVIGRFRYILEKIGPELRQYLYSNDNEEQPFSRKEYEQTVISGKYKSRMMGFGSVRDFDKPGYYIRNAMFPKQREEMHVNQTPKIETQIYKMDADNLFKRKEHAEHIKADPYFLHPDDVQVIGEHTCEKPFYVKGLVGQSAMSYGSLGE--RAVTALSKGLHL--AGGTWMNTG-EGGLSEYHLKGG-----ADIICQIGPGLFGVRKRNGEFSWEEFKRKSRIDQIKAFELKLAQGAKT-R-G----GHVDGAKVSEEVADIRNVEPGKSIDSPNRFYEFSNPPEMLDFIEKLRDVGQKPVGIKLVAGHPEELHELFSHMQKSGKHPDFITIDGSEGGTGASFYELADTVGLPIMTALPIVDTLLKQYGLRSQLKIFASGKLLTPDKIAVALALGADFVNIARGMMFSVGCIRALVCHTNTCPAGVATTDPKLQKALSVEEKQHRVCNYVISLREGLFNLAAAAGINSPIHFSKEHVVYRKEDGSII--NIDNLIHQFVS 1KBI Chain:A ((157-497)) ---------------------------------------------------------------------------------------------------------------------------------------NHNAYHRIFFKPKILVDV--RKVDISTDMLGS------HVDVPFYVSATALCKLGNPLEGEKDVARGCGQGVT-KVPQMISTLASCSPEEIIEAAPSDKQIQWYQL----YVNSDRK--I-TDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFSNT-----KKTNVEESQGASRALSK-----FIDPSLTWKDIEELKKKTKLPIVIKGVQR-----TEDVIKAA--EIGVSGVVLSNHGGRQL--------DFSRAPIEVLAETMPILEQRNLKDKLEVFVDGGVRRGTDVLKALCLGAKGVGLGRPFLYANSC-----------------------------YGRNGVEKAIEILRDEIEMSMRLLGVTSIAELKPDLLDLSTLKARTVGVPNDVLYN----

General information: TITO was launched using: RESULT: Template: 1KBI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1660 -137302 -82.71 -434.50 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -82.71 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_1KBI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KBI-query.scw PDB file : Tito_Scwrl_1KBI.pdb: