Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDITFNTGLMGSGKSKKLID-DYLIDPK-EKVALSVSLTEDTFSRGKIESRDGRSLRSINLNRDQFKQNISLLEIIIFMTNTQTIYIDESQFLPKETVEKFVSLSESYHVPIHFYGLDLTFTGELFDSSSHLLTILPSENINRISRGCEASKCSKIAQYNARIVDGKVSRSGETFVEEKSY-YLALCSDHYYNDEKII 2ORV Chain:A ((19-191)) GQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIK---YAKDTRY---------MEALPACL-LR----DVA---QEALGVAVIGIDEGQFFPD-IVEFCEAMAN-AGKTVIVAALDGTFQRKPFGAILNLVP--LAESVVKLTAVCME--CFREAAYTKRLGTE-----KEVEVIGGADKYHSVCRLCYFK-----

General information: TITO was launched using: RESULT: Template: 2ORV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 844 -89374 -105.89 -562.10 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -105.89 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_2ORV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ORV-query.scw PDB file : Tito_Scwrl_2ORV.pdb: