Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTNYWIGVV-SEQHVLKGAAGGFAQLCHGKKAPLAK-MK---E-GDWLIYYSPRDAYPDGKLLRSFTAIGKVKSGNIYPYQMA----PNFIPYRLDIDYYPCHKIGFYDIKSKLEFVQETKHLG--FLF--RRGHFEISKKDFLTIAQAMGVNISGMAL 4RCM Chain:A ((16-156)) --SRFFVIKSSSLKHVKRSFYNGIWSSTHFGNKRLSEAYKKLNSGAKVFLFFSI-------NTSGRFCGVAEMVSDLKMDLDTSIWEDEQKYGKAFKVRWVIVRDINNRSLK-RFL-----IPSNEMKPITHSRDTQEIPYSIGISIINLFKTQDS----

General information: TITO was launched using: RESULT: Template: 4RCM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 562 -78677 -139.99 -619.50 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -139.99 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.286

(partial model without unconserved sides chains): PDB file : Tito_4RCM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RCM-query.scw PDB file : Tito_Scwrl_4RCM.pdb: