Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIAFLN----SLVVTSPGFYKAEKITLDEVKQWLKHYDGRYKSFIGHKSTAQFLQKLLGIRIEQNRKTFRHMKYQKAICFSLYERYPENVLLTQRDLEKARYQFYLLTRLD 2X4I Chain:A ((2-113)) NKVYLANAFSINMLTKFPTKVVIDKIDRLEFCENID--NEDIINSIGHDSTIQLINSLCGTTFQKNRVEIKLEKEDKLYVVQISILTLEEILKLYESGKVQFFEIIV-----

General information: TITO was launched using: RESULT: Template: 2X4I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 404 -61047 -151.11 -604.42 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -151.11 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_2X4I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X4I-query.scw PDB file : Tito_Scwrl_2X4I.pdb: