Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKKRVITCVMAASLTLGSLLPAGYASAKEDSKTTPSYEELALHYKMKSEKISSNGKLVEIEYVSGN-ETHKVQMNGNNHTVKVDGIEQKGLNFEYDENVAKRTNYENNNLK----SNEFTTQAAKPKKGYHYVGTLSGHTKAAKNALSVTMSLVGIVPGLGWGSKAATILFSYWAKEQIPDAYYKYDLYEKGAMTDSWYQYATVQFFEDKAHKKKMGKPWTSTPAKVDLPNS 2FNU Chain:A ((74-184)) --RNEIITTPISFVATANMLLESGYTPVFAGIKNDGNIDELALE-KLINERTKA---IVSVDYAGKSVEVESVQKLCKKHS----------LSFLSDSSHALGSEYQNKKVGGFALASVFSFHAIKP----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FNU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 480 -10232 -21.32 -96.53 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -21.32 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.158

(partial model without unconserved sides chains): PDB file : Tito_2FNU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FNU-query.scw PDB file : Tito_Scwrl_2FNU.pdb: