Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQRVSYYEIAPEGMKIMMDMEKY-TK-QSSINRTTRELIKIRVSQMNGCAFCIDMHTSDARKMGETEQRIYCLHAWNECDFYSPEEKAALELSEHITLIPSKRVPDELYHRVRE-HYDEEQYVDLVLIINQINSWNRISIAMGNRAASK 2OYO Chain:A ((44-189)) --NVFRAQAVNGEQFLAWWNYFNLLLNKEGYLTNAERELVAVVVSGVNRCLYCAVSHGAALREFLGDPQKADAVAVNWRHADLTEREQALAAYAEKLTRHP-AEVTAADLEPLRAVGLDDHQIMELVQVIGMFNLTNRVSSALGFVPNP-

General information: TITO was launched using: RESULT: Template: 2OYO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 575 -65345 -113.64 -456.96 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -113.64 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_2OYO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OYO-query.scw PDB file : Tito_Scwrl_2OYO.pdb: