Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MN----MYWFLGALLYFLIGTYIFIR--------------VTRDSQSGSWILLALAAPL--IIAGYPYFYSKKLLSKRR 1W8X Chain:P ((1-116)) MDKKKLLYWVGGGLVLILIWLWFRNRPAAQVASNWEGPPYMTYNQPQAGSVTLPVADSITSQLNDYASSLNDYLASQAG

General information: TITO was launched using: RESULT: Template: 1W8X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 39 -5521 -141.55 -93.57 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain P : 0.72 3D Compatibility (PKB) : -141.55 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.040

(partial model without unconserved sides chains): PDB file : Tito_1W8X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W8X-query.scw PDB file : Tito_Scwrl_1W8X.pdb: