Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDFAFKLYEYNVWANQQIFNRLKELPKEIYHQEIQSVFPSISHVLSHVYLSDLGWIEVFSGKTLSDALALAEQLKEQTEAKEIEEMEDLFLRLSERYILFLQQ---KEQLNKPLQIQNPSSGIMKTTVSELLPHVVNHGTYHRGNITAMLRQAGYASAPTDYGLYLFMTKTEKA 3DI5 Chain:A ((2-150)) MYQTIEGFLQSWTYETESTQKMLDVLTDESLSQEIAPGHWTLGRVAWHIVTAIPVILSGTGL-KFEGE------TKDYPVPTSAKTIADGYRKVNTAFVEALQSEWTDKDLTTIND---FFG--RPMPNSIFLMTLINHQNHHRGQMTVLMRQAGLTVPGV--------------

General information: TITO was launched using: RESULT: Template: 3DI5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 594 -66399 -111.78 -454.79 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -111.78 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_3DI5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DI5-query.scw PDB file : Tito_Scwrl_3DI5.pdb: