Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGFSHFNEQGRAQMVDISEKSSTVRTAAAVSSVHMKNEVYEKIQSHDIGKGDVLAVAQVAGIMAAKQTSNIIPMCHPLSLSGVDISFDWKIKEAEVILHIKAQVKTKGSTGVEMEALTSASVCALTVYDMCKALDKGMVIGPTFLLEKTGGKNGDFKRELSEYNLEDQK 3JQM Chain:H ((10-155)) -----------RPRMVDVTEKPETFRTATAEAFVELTEEALSALEKGGVGKGDPLVVAQLAGILAAKKTADLIPLCHPLPLTGVEVRVE--LLKAEKRVRIEATVKTKAETGVEMEAMTACAVAALTVYDMLKAASKGLVISQVRLLHKAGGKSGEWRR-----------

General information: TITO was launched using: RESULT: Template: 3JQM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 761 -75674 -99.44 -518.32 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain H : 0.86 3D Compatibility (PKB) : -99.44 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_3JQM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JQM-query.scw PDB file : Tito_Scwrl_3JQM.pdb: