Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSDNNFVSETLENVQYLLPGAKVIKLRGYSRAHKVYTIAKSPVEKWKVAAGLSGSEIAILIRKGHWIGASIPAGGIVIDIDDS--------KQGELVKGLLDAQNFHCHCIRTL-MGGSLFLRITNMGKRKLNK 5DMU Chain:A ((135-187)) -------------------------------------------------------------------------PDRLIFDLDPPDNNFETVRSAAKTIREALDAEGYPVYLMTTGSRGLHVVVPLD---------

General information: TITO was launched using: RESULT: Template: 5DMU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 124 -25658 -206.92 -583.14 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -206.92 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_5DMU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DMU-query.scw PDB file : Tito_Scwrl_5DMU.pdb: