Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIEIIISLGIYFIAMLLIGWYAFKKTTDINDYMLGGRGLGPFVTALSAGAADMSGWMLMGVPGAMFATGLSTLWLALGLTIGAYSNYLLLAPRLRAYTEAADDAITIPDFFDKRFQHSSSLLKIVSALIIMIFFTLYTSSGMVSGGRLFESAFGA-----DYKLGLFLTTAVVVLYTLFGGFLAVSLTDFVQGAIMFAALVLVPIVAFTHVGG-VAPTFHEIDAVNPHLLDIFKGASVISII-SYLAWGLGYYGQPHIIVRFMA--------IKDIKDLKPARRIGMSWMIITVLGSVLTGLIGVAYAHKFGVAVKDPEMIFIIFSKILFHPLITGFLLSAILAAIMSSI-SSQLLVTASAVTEDLYRSFFRRKASDKELVMIGRLSVLVIAVIAVLLSLNPNSTILDLVGYAWAGFGSAFGPAILLSLYWKRMNE----------------WG----ALAAMIVGAATVLIWITTGLAKSTGVYEIIPGFILSMIAGIIVSMITKRPAKASYRLFGVMEKLLKRKK 2JLN Chain:A ((23-456)) -----------------------------------AERSVGPFSLAAIWFAMAIQVAIFIAAGQ--MTSSFQVWQVIVAIAAGCTIA-VILLFFTQSAAIRW-G-INFTVAARMPF-G-IR-GSLIPITLKALLSLFWFGFQTWLGALALDEITRLLTGFTNLPLWIVIFGAIQVVTT-FYGITFIRWMNVFASPVLLAMGVYMVYLMLDGADVSLGEVMSMGG----E----NPGMPFSTAIMIFVGGWIAVVVSIHDIVKECKVDPNASREGQTKADARYATA-QWLGMVPASIIFGFIGAASMVLVG--E---WNPVIAITEVVGG-VSIPMAILFQVFVLLATWSTNPAANLLSPAYTLCS-TFPRVFT----------FKTG-VIVSAVVGLLMMPWQFAGVLNTFLNLLASALGPLAGIMISDYFLVRRRRISLHDLYRTKGIYTYWRGVNWVALAVYAVALAVSFLTPD---------LMFVTGLIAALLLHIPAMRWVAKTFPLFS--------------

General information: TITO was launched using: RESULT: Template: 2JLN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1929 -325137 -168.55 -816.93 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -168.55 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_2JLN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JLN-query.scw PDB file : Tito_Scwrl_2JLN.pdb: