Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFGERLKKCRTSKGYSQQRMADFLGITRQG----YGKYEIGKAEPDLKTLTKLSNILGVSTDFLLKGTHAQFDLDEILNDPETLIAGYNGMISEEQAKELLYYLLKKEFEEH 3LFP Chain:A ((1-96)) MLIRRLKDARLRAGISQEKLGVLAGIDEASASARMNQYEKGKHAPDFEMANRLAKVLKIPVSYLYTPE----------DDLAQIILTWNEL-NEQERKRINFYIRKK-----

General information: TITO was launched using: RESULT: Template: 3LFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 325 8985 27.65 97.66 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 27.65 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_3LFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LFP-query.scw PDB file : Tito_Scwrl_3LFP.pdb: