Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTKTAFMAILFSLITVLSACGAGSQTTGAGQKKVQTITVGTGTQFPNICFIDEKGDLTGYDVELIKELDKRLPHYKFTFKTMEFSNLLVSLGQHKVDIVAHQMEKSKEREKKFLFNKVAYNHFPLKITVLQNNDTIRGIEDLKGKRV-ITSATSNGALVLKKWNEDNGRPFEI-AYEGQGANETANQLKSGRADAT-----------ISTPFAVDFQNKTSTIKEKTVGNVLSNAKVYFMFNKNEQTLSDDIDKALQEIIDDGTLKRLSLKWLGDDYSKEQY 1HSL Chain:A ((4-231)) -----------------------------------QKIRIGTDPTYAPFESKNAQGELVGFDIDLAKELCKRI-NTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQEIAFTDKLYAA-DSRLVVAKNSDIQPTVASLKGKRVGVLQGTTQETFGNEHWAP---KGIEIVSYQGQ--DNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPAVKDEKLFGVGTG----MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYF---------

General information: TITO was launched using: RESULT: Template: 1HSL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1151 -2984 -2.59 -13.88 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -2.59 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_1HSL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HSL-query.scw PDB file : Tito_Scwrl_1HSL.pdb: