Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATIKDIAQEAGFSISTVSRVLNNDESLSVPDETREKIYEAAEKLNYRKKTV-RPL-VKHIAFLYWLTDKEELEDVYFKTMRLEVEK----------LAKAFN--------VDMTTYKIADGIESIPEHTEGFIAVGTFSDEELAFLRNLTE-NGVFID-STPDPDHFDSVRPDLAQMTRKTVNILTEKGHKSIGFIGGTYKNPNTNQDEMDIREQTFRSYMREKAMLDERYIFCHRGFSVENGYRLMSAAIDTLGDQLPTAFMIAADPIAVGCLQALNEKGIAIPNRVSIVSINNISFAKYVSPPLTTFHIDIHELCKNAVQLLLEQVQDKRRTVKTLYVGAELIVRKSMN 1JFS Chain:A ((1-330)) -ATIKDVAKRANVSTTTVSHVIN--KTRFVAEETRNAVWAAIKELHYSPSAVARSLKVNHTKSIGLLATSSEA--AYFAEIIEAVEKNCFQKGYTLILGNAWNNLEKQRAYLSMMAQKRVDGL---------LVMCSEYPEPLLAMLEEYRHIPMVVMDFGEAKADFTDAVIDNAFEGGYMAGRYLIERGHREIGVIPGPLER-NTGAG----RLAGFMKAMEEAMIKVPESWIVQGDFEPESGYRAMQQILSQ--PHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDNVRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSV-

General information: TITO was launched using: RESULT: Template: 1JFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1600 -583 -0.36 -1.89 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -0.36 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_1JFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JFS-query.scw PDB file : Tito_Scwrl_1JFS.pdb: