Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSYMLGIDIGTTSTKAVLFSENGDVVQKESIGYPLYTPDISTAEQNPEEIFQAV--IHTTARITKQHPEKRISFISFSSAMHSVIAIDEN-DKPLTPCITWADNRSEGWAHKIKEELNGHEVYKR----TGTPIHPMAPLSKIAWITNERKEIASKAKK----YIGIKEYIFKQLFNE-----YVIDYSLASATGMMNLKGLDWDEEALRIAGITPDHLSKLVPTTEIFQHCSPEIAIQMGIDPETPFVIGASDGVLSNLGVNAIKKGEIAVTIGTSGAIRTIIDKPQTDEKGRIF---CYALTDKH--WVIGGPVNNGGIVLRWIRDEFASSEIETATRLGIDPYDVLTKIAQRVRPGSDGLLFHPYLAGERAPLWNPDVRGSFFGLTMSHKKEHMIRAALEGVIYNLYTVFLALTECMDGPVTRIQATGGFARSEVWRQMMSDIFESEVVVPESYESSCLGACILGLYATGKIDSFDAVSDMIGSTYRHTPIEDSAKEYRTLMPIFINLSRLLENQYTQIADYQRGLITHK 2D4W Chain:A ((3-482)) ---YVLAIDQGTTSSRAIVFDHSGEIYSTGQLEHDQIFPRAGWVEHNPEQIWNNVREVVGLALTRGNLTHEDIAAVGITNQRETAVVWDKTTGKPVYNAIVWQDTRTQ----KIVDELGGDEGAEKYKSIVGLPLATYFSGPKIKWILDNVEGAREKAEKGDLLFGNTDTWVLWNMTGGTEGGVHVTDVTNASRTMLMDLDTLSWREDIAADMGIPLSMLPDIRSSSEVYGHGRPR-----GLVPGVPIAGILGDQQAATFGQACFEVGQAKNTYGTGNFLLLNTGTEKVMSKNGLLTTVCYKIGDAPAVYALEGSIAVTGSLVQWLRDNLGMFE---------DAPDV-EWLAGKVQD-NGGAYFVPAFSGLFAPYWRPDARGALVGLTRYVNRNHIARAALEATAFQSREVVDAMNADSGVDLTELRVDGGMVANELLMQFQADQLGVDVVRPKVAETTALGAAYAAGIAVGFWKGEQDVIDNWAEDKRWSPSMESGERER-------------------------------

General information: TITO was launched using: RESULT: Template: 2D4W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2921 23744 8.13 51.73 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 8.13 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_2D4W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2D4W-query.scw PDB file : Tito_Scwrl_2D4W.pdb: