Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEPRKNVTDFVLLGFTQNPKEQKVLFVMFLLFYILTMVGNLLIVVTVTVSETLGSPMYFFLAGLSFIDII-----YSSSISPRLISGLFFGNNSISFQSCMAQLFIEHIFGGSEVFL--LLVMAYDCYVAICKPL--------HYLV-IMRQWVCVVLLVVSWVGGFLHSVFQLSIIYGLP-FCGPNVIDHFFCDMYPLLKLVCTDTHAIGLLVVANGGLACTIVFLLLLISYGVILHSLKNLSQKGR-----QKALSTCSSHMTVVVFFFVPCIFMYARPARTF---------PIDKSVSVFYTVITPMLNPLIYTLRNSEMTSAMKKLWRRDLISSST 4ZWJ Chain:A ((204-477)) ---------------------------MFLLIVLGFPINFLTLYVTVQ-HKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLQGFFATL-------GGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPE-----GLQCSCG--------IDYYTLKPEVNNESFVIYMFVV-----HFTIPMIIIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIYVIAFLICWVPYASVAFYIFTHQGSCFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQFRNCMLTTICCGKNVIFK

General information: TITO was launched using: RESULT: Template: 4ZWJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -251270 for 1781 contacts (-141.1/contact) + 2D Compatibility (PS) -24366 + (NN) -4235 + (LL) 3848 1D Compatibility (HY) -30000 + (ID) 3450 Total energy: -309473.0 ( -173.76 by residue) QMean score : 0.286

(partial model without unconserved sides chains): PDB file : Tito_4ZWJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZWJ-query.scw PDB file : Tito_Scwrl_4ZWJ.pdb: