Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRLHYAWIIVSVTFLILLAVQGVRLSFGAFVEPWERQFSIDRSTISLISTVSFIVYGISQPVIGRLVDKWGARAVLAWSALLTGVSIFLTYLVTSPWQLFLLYGLGVSLGVGGASNVAASVLVVNWF-SKKRGLAFGIMEAGFGAGQMLLVPGSLMLIHWFSWKLTVVVLGLLLIVIVFPAALLMLRNNPSEKNTEPIGGLAASEKETAPKTTALSVAGMFRMRQFWFLIFPFLICGFTTVGLMDTHLIPFSHD-HGFSTTVTSAAVSLLAGFNIAGILLSGIVADRWSSRKILCILYAVRALSIVILI-YS--HEPYLLLAFAILFGLVDFATVAPTQMLATQYFQNYSIGLMIGWLSLAHQIGSALGAYVPGVIYTVTGEYTLAFYLSIGMLVLASVMNVMLREPAAVTRDSAAVVDK 2GFP Chain:A ((22-360)) ----------------------MAQTIYIPAIADMARDLNVREGAVQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAAS-AMQGMGTGVG-GVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWNWRACYLFLLVLC-AGVTFSMARWMPETRPVDAP------R----T----RLLTSYKTLFGNSGFNCYLLMLIGGLAGI-AAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQSVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFP-FLAGTAGALVGGLQNIGSGVLASLSAMLPQT-----------------------------------------

General information: TITO was launched using: RESULT: Template: 2GFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1527 -215435 -141.08 -645.01 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -141.08 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_2GFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GFP-query.scw PDB file : Tito_Scwrl_2GFP.pdb: