Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATIDLQKKSVKIVLEKKQLTKVTARVGLVLDITGSMRPLYKNGTVQNVVERILAVADQFD-DNGLLDVWVYDNEFSRLKPVSEKDFSGYVDREILNNDRLHKFGRNDEPPVMKDVLRKYVTE---EPSSYPAFIVFINDGGCKK---SIKPIIEASSDKPVFWQFVGIGNG-----NFDFLNKLDTLEGRVIDNTNFLHIEEIDRISDDELYDALLAEFPFWLKEAKEKGIVREQEPPAEKPKKKGFFSRLFSK 1MF7 Chain:A ((130-317)) ---------------------QEDSDIAFLIDGSGSIIPHD-FRRMKEFVSTVM---EQLKKSKTLFSLMQYSEEFRIHFTFKEFQNNPNPRSLVKPI--TQLLGRTHTATGIRKVVRELFNITNGARKNAFKILVVITDGEKFGDPLGYEDVIPEADREGVIRYVIGVGDAFRSEKSRQELNTIASKP----PRDHVFQVNNFEALKT--IQNQLREKIF----------------------------------

General information: TITO was launched using: RESULT: Template: 1MF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 924 -100524 -108.79 -571.16 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -108.79 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_1MF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MF7-query.scw PDB file : Tito_Scwrl_1MF7.pdb: