Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKRTVKNIAEMVKGTLANPQYEQTVIHGVATDTRKLEQHQLFIPLKGERFDGHSFVEQAFEAGVAAVLWDRSVPNPPENHAVILVDDTLTALQQLAKAYLQELGTRVIGVTGSNGKTTTKDMIHAVLGTQYRVHKTGGNFNNHIGLPLTVLAMPENTEIAVLEMGMSAKGEIDLLSRLANPDAAVITNIGESHMQDLGSREGIAEAKLEIINGLKEDGVLIYIGDEPLLQNAYSCQTKTYGTGTH-NDYQLQDVSQSEEGTHFTIKG--IENTFFIPILGKHNVMNAMAAIAAGAYFGIAPEDAAKGLSGLKVTGMRLELIKTDSGLSIINDAYNASPTSMKAAIQLTESLEGYGKKMLVLGDMLELGDLEETFHKECGAVISPDKIDRVFTYGKLGAFIAEGALKHFEKDRVSHYTEK-KDLLQAVKENASKGDLILFKASRGMKLEEIVKDLIESPLS 1E0D Chain:A ((98-412)) ---------------------------------------------------------------------------------------------------FCREAQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGN----IGLPALML-LDDECELYVLEL---SSFQLETTSSL-QAVAATILNVTED----FGLQQYRA-AKLRIYENAK---VCVVNADDALTMPI--ADERCVSFGVNMGDYHLNH-QQGE--TWLRVKGEKVLNVKEMKLSGQHNYTNALAALALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALN---GLHVDGTLHLLLG-----GDGKSADFSPLARYLNGDNV-RLYCFGRDGAQLA--AL----RPEVAEQTETMEQAMRLLAPRVQPGDMVLLSPA------------------

General information: TITO was launched using: RESULT: Template: 1E0D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1779 -184809 -103.88 -609.93 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -103.88 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_1E0D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E0D-query.scw PDB file : Tito_Scwrl_1E0D.pdb: