Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLMIHSVANGLNHFCTWVMRLAYLNVLWILFSLAGLVVFGLMPATAAMFTVAREWAKGNTDAPVFSVFFRTFKKEWRASQILGLIVVTAAL--FLFADMRIAAQMDQPVLVNVFVSISLIF--------AFVVLYV-------------------FPVFSHFDVKIREVLSISFFIAFSRPAVTLLMAAGAVGVLCLVLFHVTFL-----------LFFSGSLLSLILTKLSFKAFRSM----------DQRQEKEKAA 1B35 Chain:B ((16-270)) ----ENSHIENEDKRLTSEQKEIVHFVSEGVTPSTTALPDIVNLSTNYLDKNTREDRIHSIKDFLSRPIIIATNLWSVSDPVEKQLYTANFPEVLISNAMYQDKLKGFVGLRATLVVKVQVNSQPFQQGRLMLQYIPYAQYMPNRVTLINETLQGRSGCPRTDLELSVGTEVEMRIPYVSPHLYYNLITGQGSFGSIYVVVYSQLHDQVSGTGSIEYTVWAHLEDVDVQYPTGANIFTGNEAYIKGTSRYDAAQKAHAA

General information: TITO was launched using: RESULT: Template: 1B35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 727 -164218 -225.88 -801.06 target 2D structure prediction score : 0.11 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -225.88 2D Compatibility (Sec. Struct. Predict.) : 0.11 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.082

(partial model without unconserved sides chains): PDB file : Tito_1B35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B35-query.scw PDB file : Tito_Scwrl_1B35.pdb: