Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNYLSVAVELVCGLGILFIILKLLGKTQFSQITPFDFISALILGELVGNAVYDHEIKIKEIIFASLLWGVLIYIIEFITQKMKSSRKFLEGEPNIVIRKGELQYKVMKKNKIDINQLQSLLRQAGSFSIQEVEYAILETNGMVSVLPKSDFDKPTNKDLQIPSKSVSLPITLIIDGEIVRDNLKEAGVDEQWLKQELKKKNIDKTEDVLFAEWHKNKPLYTVTYEQSRST 3VCM Chain:P ((6-43)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------TTFKRIFLKRMPSIRESLKERGVDMARLGPEWSQPMKR---------------------------

General information: TITO was launched using: RESULT: Template: 3VCM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 28 -1005 -35.89 -26.45 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain P : 0.49 3D Compatibility (PKB) : -35.89 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_3VCM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VCM-query.scw PDB file : Tito_Scwrl_3VCM.pdb: