Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------MQATVH-----ESKQSIMQRILTVF-------------VFTLLIATVGLFIGQFVPVALMLPLSILEVAMIILAFWMRRRKAVGYA--FVYTFAFVSGITLFPIVSHYASIAGAY----VVLEAFGST-----FVIFAVLGTIG--AKMKK---DLSFLWSFLLVAVLA--LAVVGIFNIFSPLNSAAMMAYSVIG-------TIVFSLYILYDLNQI--KHRHITEDLIPVMALSLYL------DFINLFINLLRFFGILSSDD------- 4N5H Chain:X ((1-315)) MADEEAMLAKVQASWAQTAARDKARYADERVPEDVHWETEYRYEQSADPQQTLNLYYPAKRRNATMPTVIDIHGGGWFYGDRNLNRNYCRYLASQG-YAVMGMGYRLLPDVDLRGQIQDIFAS-LRWLSHFGPQRGFDLDHVLLTGDS---AGGHLASLVACIQQSAELQELFGVSRVNFNFTLVALVCPVAEPSKLPEAAGDMSDMAAFYLDKLSGGDQALVDHLNFSQVVKGLDLPPFMLIGGQNDSFYLQSQALLKVFDANHVTYTTKLWPASAGPHLKHVFNVQHWEWPESIETNLEMLRTFDALSKQQDQAEENE

General information: TITO was launched using: RESULT: Template: 4N5H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1031 -217726 -211.18 -1041.75 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain X : 0.68 3D Compatibility (PKB) : -211.18 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_4N5H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N5H-query.scw PDB file : Tito_Scwrl_4N5H.pdb: